streptococcus oralis  (ATCC)

Journal: Journal of Oral Microbiology

Article Title: Functional divergence of MdpS and MdpS2 reveals mucin-targeting strategies in Streptococcus oralis

doi: 10.1080/20002297.2025.2571186

Figure Lengend Snippet: Temporal analysis of S. oralis biofilm growth and mdpS/mdpS2 mRNA expression. (A) Bacterial growth (CFU/mL) of S. oralis ATCC 9811 grown on MUC5B-coated surfaces over 48 hours. Biofilm growth increased steadily over 3 hours and continued with a minor increase up to 24 hours, followed by both conditions experiencing a 98.5% reduction between 24 and 48 hours. A solid line is used between 3 and 24 hours due to consistent CFU/mL values across replicates, while a dashed line between 24 and 48 hours reflects the variability in the decline phase. (B) Relative mRNA expression of mdpS and mdpS2 were quantified using RT-PCR using ef-tu as the reference gene, where expression was calculated using the ΔΔCt method. Data are shown as mean ± standard deviation from biological triplicates. Asterisks indicate significant differences (* p < 0.05, ** p < 0.01) compared to the 0-hour time point. Statistical analysis was performed using two-way ANOVA with Dunnett's multiple comparison test. Time 0 samples were collected prior to MUC5B exposure and were biologically equivalent across conditions. Although the RNA yield was insufficient for RT-qPCR, this time point served as a shared baseline for fold-change analysis, with a set fold change of 1 reflecting equal mRNA levels in both conditions.

Article Snippet: The subcellular localisation of MdpS2 was assessed by analysing three distinct fractions of planktonic S. oralis ATCC 9811: lysate (intracellular and cell-wall associated), surface-associated (papain-digested), and extracellular (culture supernatant).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Standard Deviation, Comparison, Quantitative RT-PCR

Journal: Journal of Oral Microbiology

Article Title: Functional divergence of MdpS and MdpS2 reveals mucin-targeting strategies in Streptococcus oralis

doi: 10.1080/20002297.2025.2571186

Figure Lengend Snippet: Enzyme-induced dispersal from S. oralis biofilms by MdpS and MdpS2. Biofilms of S. oralis ATCC 9811 were grown on MUC5B-coated surfaces and treated with MdpS, MdpS2, or buffer (control). After 24 hour treatment, viable counts (CFU/mL) were determined from both the supernatant (representing dispersed cells) and the intact (attached) biofilm, which was resuspended prior to plating. Dispersal was quantified as the ratio of supernatant CFUs to intact biofilm CFUs, normalised to the control (set to 1). Data are shown as mean ± standard deviation from technical replicates. Statistical analysis was performed using Kruskal Wallis ANOVA with Dunn's multiple comparison test (ns = p > 0.05, * p < 0.05).

Article Snippet: The subcellular localisation of MdpS2 was assessed by analysing three distinct fractions of planktonic S. oralis ATCC 9811: lysate (intracellular and cell-wall associated), surface-associated (papain-digested), and extracellular (culture supernatant).

Techniques: Control, Standard Deviation, Comparison